《植物生理学报》 2014, 50(12): 1780-1784

大麦单倍体的离体培养与快速繁殖

何婷^1,2, 陆瑞菊^1,2, 高润红^1,2, 郭桂梅^1,2, 黄亦辰^1,2,3, 杨沙沙^1,2,3, 陈志伟^1,2, 徐红卫^1,2, 李颖波^1,2, 刘成洪^1,2,*, 黄剑华^1,2,*

¹上海市农业科学院生物技术研究所, 上海201106; ²上海市农业遗传育种重点实验室, 上海201106; ³上海海洋大学水产与生命学院, 上海201306

通信作者：刘成洪；E-mail: liuchenghong@saas.sh.cn, sw1@saas.sh.cn；Tel: 021-62201032

摘　要：

以大麦品种‘花30’小孢子培养来源单倍体植株作为起始材料, 分别对其丛生芽诱导、扩繁、生根以及再生植株水培等技术进行了研究。结果表明, 添加0.5 mg•L^-1 噻苯隆(TDZ)的1/2MS培养基适宜丛生芽诱导; 添加1.0 mg•L^-1 TDZ的1/2MS培养基适宜丛生芽增殖; 分别添加400 mg•L^-1水解酪蛋白(CH)、20 mg•L^-1亚精胺(Spd)和1.0 mg•L^-1多效唑(MET)可以促进丛生芽的健壮; 添加0.05 mg•L^-1 α-萘乙酸(NAA)和1.0 mg•L^-1 MET的1/2MS培养基适宜生根。水培7~10 d后, 90%以上的再生植株正常生长。

关键词：大麦; 单倍体; 丛生芽; 快速繁殖

收稿：2014-10-15   修定：2014-12-02

资助：上海市科委基础研究重点项目(12JC1407800)、上海市种业发展项目(沪农科种字2011第1号)和国家大麦青稞产业技术体系(CARS-05)。

In Vitro Culture and Rapid Propagation of Barley Haploid

HE Ting^1,2, LU Rui-Ju^1,2, GAO Run-Hong^1,2, GUO Gui-Mei^1,2, HUANG Yi-Chen^1,2,3, YANG Sha-Sha^1,2,3, CHEN Zhi-Wei^1,2, XU Hong-Wei^1,2, LI Ying-Bo^1,2, LIU Cheng-Hong^1,2,*, HUANG Jian-Hua^1,2,*

¹Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; ²Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; ³College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: LIU Cheng-Hong; E-mail: liuchenghong@saas.sh.cn, sw1@saas.sh.cn; Tel: 021-62201032

Abstract:

In order to establish a stable barley haploid population, the haploid plants derived from microspore culture of barley (Hordeum vulgare) cultivar ‘Hua-30’ were used as materials, and the induction and rapid propagation of multiple buds, rooting and hydroponics of regenerated plantlets were studied. The results showed that the supplement of 0.5 mg•L^-1 thidiazuron (TDZ) in 1/2MS basal medium was suitable for multiple buds induction; the supplement of 1.0 mg•L^-1 TDZ in 1/2MS basal medium was suitable for bud rapid propagation and the addition of 400 mg•L^-1 casein hydrolysate (CH), 20 mg•L^-1 spermidine (Spd) and 1.0 mg•L^-1 multieffect triazole (MET) improved the state of buds growth. The supplement of 0.05 mg•L^-1 α-naphthaleneacetic acid (NAA) and 1.0 mg•L^-1 MET in 1/2MS basal medium were suitable for rooting of regenerated plantlets, and more than 90% plantlets survived after an acclimation of 7–10 days by hydroponics.

Key words: barley (Hordeum vulgare); haploid; bud; rapid propagation